Convenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique.

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

The Study of African Horse Sickness Virus by the Agar Double-Diffusion Precipitation Test l-Standardization of the Technique

Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique

Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. ...

Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.

Streitfeld, Murray M. (University of Miami, Coral Gables, Fla.), Edward M. Hoffmann, and Herbert M. Janklow. Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J. Bacteriol. 84:77-80. 1962.-Of 23 strains of P. aeruginosa isolated from pathological processes, 22 strains, representative of at least nine different somatic antigen types, were found to elaborate extracellular deo...

In Vitro Evaluation of Antifungal Activity of Three Traditionally Used Medicinal Plants; Umbilicus Intermedius Boiss, Cuminum Cyminum and Zingiber Officinale Extracts

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods. Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium...